Sample Preparation Techniques in Analytical ChemistrySomenath Mitra The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment. Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include: * State-of-the-art extraction techniques for organic and inorganic analytes * Sample preparation in biological measurements * Sample pretreatment in microscopy * Surface enhancement as a sample preparation tool in Raman and IR spectroscopy * Sample concentration and clean-up methods * Quality control steps Designed to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an invaluable reference tool for analytical chemists in the chemical, biological, pharmaceutical, environmental, and materials sciences. |
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... Electrophoretic Separation of Nucleic Acids 317 318 318 319 323 324 326 328 329 331 331 333 335 339 342 344 345 346 347 348 351 352 355 360 8.6.1. Gel Electrophoresis for Nucleic Acids Purification 360 8.6.2. Techniques xiv contents.
... Electrophoretic Separation of Nucleic Acids 317 318 318 319 323 324 326 328 329 331 331 333 335 339 342 344 345 346 347 348 351 352 355 360 8.6.1. Gel Electrophoresis for Nucleic Acids Purification 360 8.6.2. Techniques xiv contents.
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Somenath Mitra. 8.6.1. Gel Electrophoresis for Nucleic Acids Purification 360 8.6.2. Techniques for the Isolation of DNA from Gels 362 8.7. Capillary Electrophoresis for Sequencing and Sizing 364 8.8. Microfabricated Devices for Nucleic ...
Somenath Mitra. 8.6.1. Gel Electrophoresis for Nucleic Acids Purification 360 8.6.2. Techniques for the Isolation of DNA from Gels 362 8.7. Capillary Electrophoresis for Sequencing and Sizing 364 8.8. Microfabricated Devices for Nucleic ...
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... electrophoresis for DNA sequencing, and electron microscopy for small structures. Common analytical instrumentation and the sample preparation associated with them are listed in Table 1.1. The sample preparation depends on the ...
... electrophoresis for DNA sequencing, and electron microscopy for small structures. Common analytical instrumentation and the sample preparation associated with them are listed in Table 1.1. The sample preparation depends on the ...
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... Electrophoresis, UV-VIS, florescence Amino acids, fats carbohydrates Extraction, cleanup GC, HPLC, electrophoresis Microstructures Etching, polishing, reac- Microscopy, surface spectrostive ion techniques, ion copy bombardments, etc ...
... Electrophoresis, UV-VIS, florescence Amino acids, fats carbohydrates Extraction, cleanup GC, HPLC, electrophoresis Microstructures Etching, polishing, reac- Microscopy, surface spectrostive ion techniques, ion copy bombardments, etc ...
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... electrophoresis of recombinant DNA, may consist of the following steps: 1. Compare precision by analyzing multiple (say, six) independent replicates of reference standards under identical conditions. 2. Data should be analyzed with a ...
... electrophoresis of recombinant DNA, may consist of the following steps: 1. Compare precision by analyzing multiple (say, six) independent replicates of reference standards under identical conditions. 2. Data should be analyzed with a ...
Contents
1 | |
SECTION A EXTRACTION AND ENRICHMENT IN SAMPLE PREPARATION | 37 |
CHAPTER 3 EXTRACTION OF SEMIVOLATILE ORGANIC COMPOUNDS FROM SOLID MATRICES | 139 |
CHAPTER 4 EXTRACTION OF VOLATILE ORGANIC COMPOUNDS FROM SOLIDS AND LIQUIDS | 183 |
CHAPTER 5 PREPARATION OF SAMPLES FOR METALS ANALYSIS | 227 |
SECTION B SAMPLE PREPARATION FOR NUCLEIC ACID ANALYSIS | 271 |
CHAPTER 7 SAMPLE PREPARATION IN RNA ANALYSIS | 301 |
CHAPTER 8 TECHNIQUES FOR THE EXTRACTION ISOLATION AND PURIFICATION OF NUCLEIC ACIDS | 331 |
SECTION C SAMPLE PREPARATION IN MICROSCOPY AND SPECTROSCOPY | 377 |
CHAPTER 10 SURFACE ENHANCEMENT BY SAMPLE AND SUBSTRATE PREPARATION TECHNIQUES IN RAMAN AND INFRARED SPEC... | 413 |
INDEX | 439 |
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Common terms and phrases
acid added addition allows amount Anal analysis analyte applications approach aqueous bonded bu¤er cell centrifugation Chem chemical chromatography coating column common complex compounds concentration containing depends described detection determined di¤erent digestion dissolved e¤ect electron elution ethanol example extraction fiber Figure followed groups headspace heating hydrophobic important increases injection interactions involves isolation layer less limit liquid materials matrix measurement membrane metals method minutes molecules needs obtained organic particles permission phase phenol plasmid polar possible precipitation present pressure procedure produce Raman range reaction recovery reduced referred relatively removed retained sample preparation selective separation shown silica soil solid solubility solution solvent sorbent species specimen SPME standard step surface Table techniques temperature tion tissue transfer trap tube typically vapor volatile volume
Popular passages
Page 328 - A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 24. Sambrook, J., Fritsch, EF, and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual.
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Page i - A complete list of the titles in this series appears at the end of this volume.
Page 134 - Coordinating supercritical fluid and solidphase extraction with chromatographic and immunoassay analysis of herbicides, in MT Meyer and EM Thurman, eds., Herbicide Metabolites in Surface Water and Groundwater, ACS Symposium Series 630, American Chemical Society, Washington, DC, 1996, pp.
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