Sample Preparation Techniques in Analytical ChemistrySomenath Mitra The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment. Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include: * State-of-the-art extraction techniques for organic and inorganic analytes * Sample preparation in biological measurements * Sample pretreatment in microscopy * Surface enhancement as a sample preparation tool in Raman and IR spectroscopy * Sample concentration and clean-up methods * Quality control steps Designed to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an invaluable reference tool for analytical chemists in the chemical, biological, pharmaceutical, environmental, and materials sciences. |
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... Examples of RNA Isolation Using Phenol Extraction Guanidinium Salt Method 7.4.1. Examples of RNA Isolation Using Guanidinium Salts Isolation of RNA from Nuclear and Cytoplasmic Cellular Fractions 288 288 289 290 290 291 291 294 296 296 ...
... Examples of RNA Isolation Using Phenol Extraction Guanidinium Salt Method 7.4.1. Examples of RNA Isolation Using Guanidinium Salts Isolation of RNA from Nuclear and Cytoplasmic Cellular Fractions 288 288 289 290 290 291 291 294 296 296 ...
Page xx
... examples of DNA and RNA analyses are presented, along with the description of techniques used in these procedures. Sections on highthroughput workstations and microfabricated devices are included. The third section deals with sample ...
... examples of DNA and RNA analyses are presented, along with the description of techniques used in these procedures. Sections on highthroughput workstations and microfabricated devices are included. The third section deals with sample ...
Page 1
... example, while collecting samples for determination of Ca2þ in a lake, it should be kept in mind that its concentrations can vary depending on the location, the depth, and the time of year. The next step is sample preservation. This is ...
... example, while collecting samples for determination of Ca2þ in a lake, it should be kept in mind that its concentrations can vary depending on the location, the depth, and the time of year. The next step is sample preservation. This is ...
Page 2
... example, in the analysis of pesticides in fish liver, it is not possible to analyze the liver directly. The pesticides have to be extracted into a solution, which can be analyzed by an instrument. There might be several processes within ...
... example, in the analysis of pesticides in fish liver, it is not possible to analyze the liver directly. The pesticides have to be extracted into a solution, which can be analyzed by an instrument. There might be several processes within ...
Page 3
... example, the presence of pesticide in fish is a topic of concern. The questions may be: Are there pesticides in fish? If so, which ones? An analysis designed to address these questions is a qualitative analysis, where the analyst ...
... example, the presence of pesticide in fish is a topic of concern. The questions may be: Are there pesticides in fish? If so, which ones? An analysis designed to address these questions is a qualitative analysis, where the analyst ...
Contents
1 | |
SECTION A EXTRACTION AND ENRICHMENT IN SAMPLE PREPARATION | 37 |
CHAPTER 3 EXTRACTION OF SEMIVOLATILE ORGANIC COMPOUNDS FROM SOLID MATRICES | 139 |
CHAPTER 4 EXTRACTION OF VOLATILE ORGANIC COMPOUNDS FROM SOLIDS AND LIQUIDS | 183 |
CHAPTER 5 PREPARATION OF SAMPLES FOR METALS ANALYSIS | 227 |
SECTION B SAMPLE PREPARATION FOR NUCLEIC ACID ANALYSIS | 271 |
CHAPTER 7 SAMPLE PREPARATION IN RNA ANALYSIS | 301 |
CHAPTER 8 TECHNIQUES FOR THE EXTRACTION ISOLATION AND PURIFICATION OF NUCLEIC ACIDS | 331 |
SECTION C SAMPLE PREPARATION IN MICROSCOPY AND SPECTROSCOPY | 377 |
CHAPTER 10 SURFACE ENHANCEMENT BY SAMPLE AND SUBSTRATE PREPARATION TECHNIQUES IN RAMAN AND INFRARED SPEC... | 413 |
INDEX | 439 |
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Common terms and phrases
acid added addition allows amount Anal analysis analyte applications approach aqueous bonded bu¤er cell centrifugation Chem chemical chromatography coating column common complex compounds concentration containing depends described detection determined di¤erent digestion dissolved e¤ect electron elution ethanol example extraction fiber Figure followed groups headspace heating hydrophobic important increases injection interactions involves isolation layer less limit liquid materials matrix measurement membrane metals method minutes molecules needs obtained organic particles permission phase phenol plasmid polar possible precipitation present pressure procedure produce Raman range reaction recovery reduced referred relatively removed retained sample preparation selective separation shown silica soil solid solubility solution solvent sorbent species specimen SPME standard step surface Table techniques temperature tion tissue transfer trap tube typically vapor volatile volume
Popular passages
Page 328 - A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 24. Sambrook, J., Fritsch, EF, and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual.
Page 328 - FM Ausubel, R. Brent, RE Kingston, DD Moore, JG Seidman, JA Smith, and K. Struhl, Current Protocols in Molecular Biology, John Wiley and Sons, Inc., New York, 1995.
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Page 131 - The Hydrophobic Effect: Formation of Micelles and Biological Membranes', Wiley, New York, 1980.
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Page i - A complete list of the titles in this series appears at the end of this volume.
Page 134 - Coordinating supercritical fluid and solidphase extraction with chromatographic and immunoassay analysis of herbicides, in MT Meyer and EM Thurman, eds., Herbicide Metabolites in Surface Water and Groundwater, ACS Symposium Series 630, American Chemical Society, Washington, DC, 1996, pp.
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