Sample Preparation Techniques in Analytical ChemistrySomenath Mitra The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment. Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include: * State-of-the-art extraction techniques for organic and inorganic analytes * Sample preparation in biological measurements * Sample pretreatment in microscopy * Surface enhancement as a sample preparation tool in Raman and IR spectroscopy * Sample concentration and clean-up methods * Quality control steps Designed to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an invaluable reference tool for analytical chemists in the chemical, biological, pharmaceutical, environmental, and materials sciences. |
From inside the book
Results 1-5 of 95
Page viii
... ORGANICS FROM LIQUIDS Martha J. M. Wells 2.1. 2.2. 2.3. 2.4. Principles of Extraction 2.1.1. Volatilization 2.1.2. Hydrophobicity 2.1.3. Acid–Base Equilibria 2.1.4. Distribution of Hydrophobic Ionogenic Organic Compounds Liquid–Liquid ...
... ORGANICS FROM LIQUIDS Martha J. M. Wells 2.1. 2.2. 2.3. 2.4. Principles of Extraction 2.1.1. Volatilization 2.1.2. Hydrophobicity 2.1.3. Acid–Base Equilibria 2.1.4. Distribution of Hydrophobic Ionogenic Organic Compounds Liquid–Liquid ...
Page x
... ORGANIC COMPOUNDS FROM SOLIDS AND LIQUIDS Gregory C. Slack, Nicholas H. Snow, and Dawen Kou 4.1. 4.2. 4.3. 4.4. Volatile Organics and Their Analysis Static Headspace Extraction 4.2.1. Sample Preparation for Static Headspace Extraction ...
... ORGANIC COMPOUNDS FROM SOLIDS AND LIQUIDS Gregory C. Slack, Nicholas H. Snow, and Dawen Kou 4.1. 4.2. 4.3. 4.4. Volatile Organics and Their Analysis Static Headspace Extraction 4.2.1. Sample Preparation for Static Headspace Extraction ...
Page xi
... Organics Choosing an SPME Fiber Coating 4.4.3. Optimizing Extraction Conditions 4.4.4. Optimizing SPME–GC Injection ... Organic Extraction of Metals 5.3.2. Extraction with Supercritical Fluids 5.3.3. Ultrasonic Sample Preparation 201 ...
... Organics Choosing an SPME Fiber Coating 4.4.3. Optimizing Extraction Conditions 4.4.4. Optimizing SPME–GC Injection ... Organic Extraction of Metals 5.3.2. Extraction with Supercritical Fluids 5.3.3. Ultrasonic Sample Preparation 201 ...
Page 5
... organic content. Calibration Curves The most common calibration method is to prepare standards of known concentrations, covering the concentration range expected in the sample. The matrix of the standard should be as close to the ...
... organic content. Calibration Curves The most common calibration method is to prepare standards of known concentrations, covering the concentration range expected in the sample. The matrix of the standard should be as close to the ...
Page 18
... Organics Organic carbon Purgeable hydrocarbons Purgeable aromatics PCBs Organics in soil Fish tissues Biochemical oxygen demand Chemical oxygen demand None None Cool to 4 C Cool to 4 C Cool to 4 C, add zinc acetate and NaOH to pH 9 ...
... Organics Organic carbon Purgeable hydrocarbons Purgeable aromatics PCBs Organics in soil Fish tissues Biochemical oxygen demand Chemical oxygen demand None None Cool to 4 C Cool to 4 C Cool to 4 C, add zinc acetate and NaOH to pH 9 ...
Contents
1 | |
SECTION A EXTRACTION AND ENRICHMENT IN SAMPLE PREPARATION | 37 |
CHAPTER 3 EXTRACTION OF SEMIVOLATILE ORGANIC COMPOUNDS FROM SOLID MATRICES | 139 |
CHAPTER 4 EXTRACTION OF VOLATILE ORGANIC COMPOUNDS FROM SOLIDS AND LIQUIDS | 183 |
CHAPTER 5 PREPARATION OF SAMPLES FOR METALS ANALYSIS | 227 |
SECTION B SAMPLE PREPARATION FOR NUCLEIC ACID ANALYSIS | 271 |
CHAPTER 7 SAMPLE PREPARATION IN RNA ANALYSIS | 301 |
CHAPTER 8 TECHNIQUES FOR THE EXTRACTION ISOLATION AND PURIFICATION OF NUCLEIC ACIDS | 331 |
SECTION C SAMPLE PREPARATION IN MICROSCOPY AND SPECTROSCOPY | 377 |
CHAPTER 10 SURFACE ENHANCEMENT BY SAMPLE AND SUBSTRATE PREPARATION TECHNIQUES IN RAMAN AND INFRARED SPEC... | 413 |
INDEX | 439 |
Other editions - View all
Common terms and phrases
acid added addition allows amount Anal analysis analyte applications approach aqueous bonded bu¤er cell centrifugation Chem chemical chromatography coating column common complex compounds concentration containing depends described detection determined di¤erent digestion dissolved e¤ect electron elution ethanol example extraction fiber Figure followed groups headspace heating hydrophobic important increases injection interactions involves isolation layer less limit liquid materials matrix measurement membrane metals method minutes molecules needs obtained organic particles permission phase phenol plasmid polar possible precipitation present pressure procedure produce Raman range reaction recovery reduced referred relatively removed retained sample preparation selective separation shown silica soil solid solubility solution solvent sorbent species specimen SPME standard step surface Table techniques temperature tion tissue transfer trap tube typically vapor volatile volume
Popular passages
Page 328 - A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 24. Sambrook, J., Fritsch, EF, and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual.
Page 328 - FM Ausubel, R. Brent, RE Kingston, DD Moore, JG Seidman, JA Smith, and K. Struhl, Current Protocols in Molecular Biology, John Wiley and Sons, Inc., New York, 1995.
Page 182 - Volatile organic compounds (VOC) means any compound of carbon, excluding carbon monoxide, carbon dioxide, carbonic acid, metallic carbides or carbonates, and ammonium carbonate, which participates in atmospheric photochemical reactions.
Page 299 - Saiki, RK, DH Gelfand, S. Stoffel, SJ Scharf, R. Higuchi, GT Horn, KB Mullis, and HA Erlich. 1988. Primerdirected enzymatic amplification of DNA with a thermostable DNA polymerase.
Page 131 - The Hydrophobic Effect: Formation of Micelles and Biological Membranes', Wiley, New York, 1980.
Page 328 - PCR Primer: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1995.
Page 97 - Method selection guide for the isolation of organic compounds from solution. SAX = strong anion exchanger; SCX = strong cation exchanger; WCX = weak cation exchanger; RP = reversed-phase sampling conditions; NP = normal-phase sampling conditions; IE = ionexchange sampling conditions.
Page i - A complete list of the titles in this series appears at the end of this volume.
Page 134 - Coordinating supercritical fluid and solidphase extraction with chromatographic and immunoassay analysis of herbicides, in MT Meyer and EM Thurman, eds., Herbicide Metabolites in Surface Water and Groundwater, ACS Symposium Series 630, American Chemical Society, Washington, DC, 1996, pp.
Page xvi - Chemistry and Environmental Science New Jersey Institute of Technology Newark, New Jersey...