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Electron Microscopy ROGER M COLE AND TERRY J POPKIN
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture cytoplasmic described determine dilution Dissolve distilled water effective electron enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method microscopy mixture mutants needed obtained organisms oxygen plasmid plate positive prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York