Manual of Methods for General Bacteriology |
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Page 26
... Gram Staining Gram staining is the most important differ- ential technique applied to bacteria . In theory , it should be possible to divide bacteria into two groups , gram positive and gram negative ; in practice , there are instances ...
... Gram Staining Gram staining is the most important differ- ential technique applied to bacteria . In theory , it should be possible to divide bacteria into two groups , gram positive and gram negative ; in practice , there are instances ...
Page 27
... gram - positive bacteria appear blue or violet and gram - negative bacteria appear red . Gram - negative bacteria ... stain- ing . Carbolfuchsin stain : Basic fuchsin , 0.3 g Ethanol , 95 % ( vol / vol ) , 10 ml Phenol , heat - melted ...
... gram - positive bacteria appear blue or violet and gram - negative bacteria appear red . Gram - negative bacteria ... stain- ing . Carbolfuchsin stain : Basic fuchsin , 0.3 g Ethanol , 95 % ( vol / vol ) , 10 ml Phenol , heat - melted ...
Page 32
... Gram stain , rickettsiae appear gram nega- tive . Giemsa method for staining rickettsiae . Stock solution : Giemsa powder , 0.5 g Glycerol , 33 ml Absolute methanol ( acetone - free ) , 33 ml Dissolve the Giemsa powder in the glycerol ...
... Gram stain , rickettsiae appear gram nega- tive . Giemsa method for staining rickettsiae . Stock solution : Giemsa powder , 0.5 g Glycerol , 33 ml Absolute methanol ( acetone - free ) , 33 ml Dissolve the Giemsa powder in the glycerol ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes