Manual of Methods for General Bacteriology |
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Page 76
... pipette used for transferring me- dium with the gas prior to drawing medium into it . Use a rubber mouth tube ... Pasteur pipettes with sterile gas prior to transfer . Transfer can also be made with stainless - steel or platinum loops ...
... pipette used for transferring me- dium with the gas prior to drawing medium into it . Use a rubber mouth tube ... Pasteur pipettes with sterile gas prior to transfer . Transfer can also be made with stainless - steel or platinum loops ...
Page 129
... Pasteur pipette . Another disadvantage is that the bacteria to be isolated must be able to withstand temporarily the 45 to A 50 ° C temperature of the molten agar . Chapter 9 Solid Culture NOEL R. KRIEG AND PHILIPP GERHARDT. 129 B C D ...
... Pasteur pipette . Another disadvantage is that the bacteria to be isolated must be able to withstand temporarily the 45 to A 50 ° C temperature of the molten agar . Chapter 9 Solid Culture NOEL R. KRIEG AND PHILIPP GERHARDT. 129 B C D ...
Page 183
... Pasteur pipette . 3. Carry out a final single dilution of the sam- ple in a ratio of at least 1 : 1 with 0.1 N HCl . This kills the bacteria and gives their surface a net positive charge so they will not aggregate but will adsorb onto ...
... Pasteur pipette . 3. Carry out a final single dilution of the sam- ple in a ratio of at least 1 : 1 with 0.1 N HCl . This kills the bacteria and gives their surface a net positive charge so they will not aggregate but will adsorb onto ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes