Manual of Methods for General Bacteriology |
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Page 312
... activity fraction . We can estimate that the total activity is distributed in the five fractions as 5 , 20 , 50 , 20 , and 5 % ; hence , the count rate in each 0.2 - ml sample should be 500 , 2,000 , 5,000 , 2,000 , and 500 cpm , or ...
... activity fraction . We can estimate that the total activity is distributed in the five fractions as 5 , 20 , 50 , 20 , and 5 % ; hence , the count rate in each 0.2 - ml sample should be 500 , 2,000 , 5,000 , 2,000 , and 500 cpm , or ...
Page 370
... ACTIVITY 18.2.1 . General Considerations An enzyme assay should , if possible , be accu- rate , sensitive , continuous , convenient , and spe- cific for the reaction under study . More often than not , however , less ideal conditions ...
... ACTIVITY 18.2.1 . General Considerations An enzyme assay should , if possible , be accu- rate , sensitive , continuous , convenient , and spe- cific for the reaction under study . More often than not , however , less ideal conditions ...
Page 373
... activity versus biomass . Prepare another series of reaction mixtures in which the amount of cells added to the tubes is incrementally varied from zero to relatively high levels . At the higher cell levels any turbidity ( A420 ) caused ...
... activity versus biomass . Prepare another series of reaction mixtures in which the amount of cells added to the tubes is incrementally varied from zero to relatively high levels . At the higher cell levels any turbidity ( A420 ) caused ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes