Manual of Methods for General Bacteriology |
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Page 143
... Agar Agar is extracted from certain red marine algae and is the most commonly used solidifying agent for bacteriological media . Agar consists of two polysaccharides , agarose and agaropectin , with the former comprising about 70 % of ...
... Agar Agar is extracted from certain red marine algae and is the most commonly used solidifying agent for bacteriological media . Agar consists of two polysaccharides , agarose and agaropectin , with the former comprising about 70 % of ...
Page 144
... agar to a liquid basal me- dium , heat the mixture to boiling to dissolve the agar completely . If heated over a flame or on a hot plate , stir the medium constantly during heating to prevent the agar from settling to the bottom of the ...
... agar to a liquid basal me- dium , heat the mixture to boiling to dissolve the agar completely . If heated over a flame or on a hot plate , stir the medium constantly during heating to prevent the agar from settling to the bottom of the ...
Page 188
... agar while the plate rests on this level surface . Prepare small test tubes ( 13 by 100 mm ) with 2.5 to 3 ml of soft agar ( 0.7 % ) . It is convenient to prepare stock bottles with this strength agar in your basal medium . Melt the agar ...
... agar while the plate rests on this level surface . Prepare small test tubes ( 13 by 100 mm ) with 2.5 to 3 ml of soft agar ( 0.7 % ) . It is convenient to prepare stock bottles with this strength agar in your basal medium . Melt the agar ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes