Manual of Methods for General Bacteriology |
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Page 120
Use this suspension to inoculate concentration of 0.001 % , as in mitis - salivarius
suitable culture media ( e.g. , 8.5.26 ) . agar ( 8.5.31 ) . S. mitis forms tiny blue
colonies , S. salivarius forms larger blue “ gum - drop " col8.2.2 . Alkaline pH ...
Use this suspension to inoculate concentration of 0.001 % , as in mitis - salivarius
suitable culture media ( e.g. , 8.5.26 ) . agar ( 8.5.31 ) . S. mitis forms tiny blue
colonies , S. salivarius forms larger blue “ gum - drop " col8.2.2 . Alkaline pH ...
Page 132
Boil to dissolve agar . Sterilize Ferric citrate at 121 ° C for 15 min . Distilled water
100 ml 8.5.13 . Brilliant Green Lactose Bile Broth Combine the three solutions
and heat to 100 ° C ( available from BBL or Difco as Brilliant for 10 min . Sterilize
at ...
Boil to dissolve agar . Sterilize Ferric citrate at 121 ° C for 15 min . Distilled water
100 ml 8.5.13 . Brilliant Green Lactose Bile Broth Combine the three solutions
and heat to 100 ° C ( available from BBL or Difco as Brilliant for 10 min . Sterilize
at ...
Page 143
Agar .2 . Carrageenan .3 . Silica Gel 9.2 . SOLID CULTURE TECHNIQUES 9.2.1 .
Solid - Surface Mass Culture .2 . ... firm gels , have been used in bacteriology
since position and structure of agar , see references 4 adopted by Robert Koch .
Agar .2 . Carrageenan .3 . Silica Gel 9.2 . SOLID CULTURE TECHNIQUES 9.2.1 .
Solid - Surface Mass Culture .2 . ... firm gels , have been used in bacteriology
since position and structure of agar , see references 4 adopted by Robert Koch .
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Contents
MORPHOLOGY | 5 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plate positive prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York