Manual of Methods for General Bacteriology |
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Page 41
3. Wash the pellet twice in Kellenberger 4. Replace with 1.0 ml of fresh 100 %
ethanol . Veronal - acetate buffer , centrifuging between Add 1.0 ml of complete
Spurr's medium , and washes . mix thoroughly but gently . Allow to stand , with 4.
3. Wash the pellet twice in Kellenberger 4. Replace with 1.0 ml of fresh 100 %
ethanol . Veronal - acetate buffer , centrifuging between Add 1.0 ml of complete
Spurr's medium , and washes . mix thoroughly but gently . Allow to stand , with 4.
Page 128
Flame - sterilize the loop and allow it to However , one often needs to dilute the
sample cool ( 15 s ) . ( C ) Draw the loop over section I as shown , in order to
obtain well - isolated colonies , and the and immediately streak back and forth
over ...
Flame - sterilize the loop and allow it to However , one often needs to dilute the
sample cool ( 15 s ) . ( C ) Draw the loop over section I as shown , in order to
obtain well - isolated colonies , and the and immediately streak back and forth
over ...
Page 431
Dip the end of the capillary tube into the antigen extract , and remove the
forefinger to allow a rise of 2 to 3 cm of antigen . There must be no air space
between the antiserum and the extract in the capillary as the extract rises .
Replace the ...
Dip the end of the capillary tube into the antigen extract , and remove the
forefinger to allow a rise of 2 to 3 cm of antigen . There must be no air space
between the antiserum and the extract in the capillary as the extract rises .
Replace the ...
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Contents
MORPHOLOGY | 5 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plate positive prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York