Manual of Methods for General Bacteriology |
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Page 24
... aqueous OsO , fumes for 2 to 3 min . But it is much better to spread a loopful of cell suspension over the surface of a block of sterile agar and then fix the cells in an appropriate fashion after the fluid has soaked into the agar ...
... aqueous OsO , fumes for 2 to 3 min . But it is much better to spread a loopful of cell suspension over the surface of a block of sterile agar and then fix the cells in an appropriate fashion after the fluid has soaked into the agar ...
Page 29
... aqueous solution of crystal violet . 2. Wash off the dye with a 20 % ( wt / vol ) aqueous solution of copper sulfate ( CuSO4 • 5H2O ) , drain , and blot dry . Capsules appear light blue , and organisms are dark purple . 3.3.10 ...
... aqueous solution of crystal violet . 2. Wash off the dye with a 20 % ( wt / vol ) aqueous solution of copper sulfate ( CuSO4 • 5H2O ) , drain , and blot dry . Capsules appear light blue , and organisms are dark purple . 3.3.10 ...
Page 31
... aqueous am- monium hydroxide ( drops ) to a 0.5 % ( wt / vol ) aqueous solution of silver nitrate until the precip- itate which initially forms just redissolves . Solution D : Ethanol , absolute Procedure : 1. Using slides cleaned in ...
... aqueous am- monium hydroxide ( drops ) to a 0.5 % ( wt / vol ) aqueous solution of silver nitrate until the precip- itate which initially forms just redissolves . Solution D : Ethanol , absolute Procedure : 1. Using slides cleaned in ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes