Manual of Methods for General Bacteriology |
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Page 82
Philipp Gerhardt. above are autoclaved , the solutions should be filter sterilized or autoclaved separately at a pH < 5.0 ... autoclaving if sufficient pyridoxine is included in the medium . A few bacteria require pyridoxamine 5 ' - phos ...
Philipp Gerhardt. above are autoclaved , the solutions should be filter sterilized or autoclaved separately at a pH < 5.0 ... autoclaving if sufficient pyridoxine is included in the medium . A few bacteria require pyridoxamine 5 ' - phos ...
Page 164
... autoclaving . After sterilization , several carboys can be connected in series to increase the reservoir volume without changing the reservoir ; this is accomplished by connecting line A of the first reservoir to line B of the adjacent ...
... autoclaving . After sterilization , several carboys can be connected in series to increase the reservoir volume without changing the reservoir ; this is accomplished by connecting line A of the first reservoir to line B of the adjacent ...
Page 436
... autoclave in a press for 15 min using fast exhaust . After autoclaving , mix by inverting the tubes in the press several times . any tubes that develop a light pink color ( indi- cating oxidation ) upon standing . 20.3.29 . Seawater ...
... autoclave in a press for 15 min using fast exhaust . After autoclaving , mix by inverting the tubes in the press several times . any tubes that develop a light pink color ( indi- cating oxidation ) upon standing . 20.3.29 . Seawater ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes