Manual of Methods for General Bacteriology |
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Page 130
... clone devel- ops ; then remove some of the cells of the clone with a ' capillary pipette . Since the growth con- ditions for the clone are semianaerobic , this pro- cedure may not work for strictly aerobic orga- nisms . 8.5 . MEDIA AND ...
... clone devel- ops ; then remove some of the cells of the clone with a ' capillary pipette . Since the growth con- ditions for the clone are semianaerobic , this pro- cedure may not work for strictly aerobic orga- nisms . 8.5 . MEDIA AND ...
Page 234
... clones to supple- mented minimal agar plates ( 13.9.10 or 13.9.15 ) containing the nine combinations of metabolic ... clone further by streaking it out on a minimal agar plate supple- mented with the suspected growth requirement to ...
... clones to supple- mented minimal agar plates ( 13.9.10 or 13.9.15 ) containing the nine combinations of metabolic ... clone further by streaking it out on a minimal agar plate supple- mented with the suspected growth requirement to ...
Page 282
... cloning of DNA from F sex factor of Escherichia coli K - 12 Proc . Natl . Acad . Sci . U.S.A. 73 : 64-68 . 28. Southern , E. M. 1975. Detection of specific se- quences among DNA fragments separated by gel electrophoresis . J. Mol . Biol ...
... cloning of DNA from F sex factor of Escherichia coli K - 12 Proc . Natl . Acad . Sci . U.S.A. 73 : 64-68 . 28. Southern , E. M. 1975. Detection of specific se- quences among DNA fragments separated by gel electrophoresis . J. Mol . Biol ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes