Manual of Methods for General Bacteriology |
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Page 221
... described in this section of the Manual . In chapter 13 there are described various techniques for inducing , selecting , and characterizing bacterial mutants of the two most frequently used bacteria in genetic analysis , E. coli and ...
... described in this section of the Manual . In chapter 13 there are described various techniques for inducing , selecting , and characterizing bacterial mutants of the two most frequently used bacteria in genetic analysis , E. coli and ...
Page 332
... described in the first step of the lipid fractionation . Transfer 1 ml into a 15 by 100 mm test tube . Dilute with 1 ml of water to give a trichloroacetic acid concentration of 5 % ( wt / vol ) . 2. Heat the diluted sample for 30 min at ...
... described in the first step of the lipid fractionation . Transfer 1 ml into a 15 by 100 mm test tube . Dilute with 1 ml of water to give a trichloroacetic acid concentration of 5 % ( wt / vol ) . 2. Heat the diluted sample for 30 min at ...
Page 338
... described by Dische ( 7 ) can be used to detect deoxyhexoses and deoxypentoses . The modification of Osborn ( 13 ) , described here , is a rather specific method for the determination of heptoses in purified preparations of glycopro ...
... described by Dische ( 7 ) can be used to detect deoxyhexoses and deoxypentoses . The modification of Osborn ( 13 ) , described here , is a rather specific method for the determination of heptoses in purified preparations of glycopro ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes