Manual of Methods for General Bacteriology |
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Page 29
... examine the wet preparation . Vegetative azoto- bacters are yellowish green ; early encystment stages appear with a darker green cytoplasm somewhat receded from the outer cell wall , from which it is divided by a brownish - red layer ...
... examine the wet preparation . Vegetative azoto- bacters are yellowish green ; early encystment stages appear with a darker green cytoplasm somewhat receded from the outer cell wall , from which it is divided by a brownish - red layer ...
Page 31
... examine . Polysaccharides stain red , and other cytoplas- mic components are green . Alcian blue method for staining polysac- charide . Alcian blue also may be used to reveal poly- saccharides . A 1 % ( wt / vol ) solution of Alcian ...
... examine . Polysaccharides stain red , and other cytoplas- mic components are green . Alcian blue method for staining polysac- charide . Alcian blue also may be used to reveal poly- saccharides . A 1 % ( wt / vol ) solution of Alcian ...
Page 32
... examine for the presence of basophilic intracytoplasmic organisms . Gimenez method for staining rickettsiae . Stock solution : Basic fuchsin , 10 g Ethanol , 95 % ( vol / vol ) , 100 ml Phenol , 4 % ( wt / vol ) aqueous , 250 ml ...
... examine for the presence of basophilic intracytoplasmic organisms . Gimenez method for staining rickettsiae . Stock solution : Basic fuchsin , 10 g Ethanol , 95 % ( vol / vol ) , 100 ml Phenol , 4 % ( wt / vol ) aqueous , 250 ml ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes