Manual of Methods for General Bacteriology |
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Page 60
In some cases , this non - sedito determine the subcellular localization of an
mentable membrane fraction can represent as enzyme or protein in E. coli or
other enteric much as 20 to 30 % of the cytoplasmic membrane . bacteria . This
scheme ...
In some cases , this non - sedito determine the subcellular localization of an
mentable membrane fraction can represent as enzyme or protein in E. coli or
other enteric much as 20 to 30 % of the cytoplasmic membrane . bacteria . This
scheme ...
Page 329
The precipita fraction of macromolecules is sequentially extracted with organic
solvents for lipids , alkali for ribonucleic acids ( RNAs ) , and hot trichloroacetic
acid for deoxyribonucleic acids ( DNAs ) ; the precipitate remaining after these ...
The precipita fraction of macromolecules is sequentially extracted with organic
solvents for lipids , alkali for ribonucleic acids ( RNAs ) , and hot trichloroacetic
acid for deoxyribonucleic acids ( DNAs ) ; the precipitate remaining after these ...
Page 331
Determine the radioactivity of the hydroml of ice - cold 70 % ethanol to remove
residual lyzed RNA fraction in the same way as for the trichloroacetic acid , which
may cause solubili small - molecule fraction ( see above and 16.4.3 ) . zation of ...
Determine the radioactivity of the hydroml of ice - cold 70 % ethanol to remove
residual lyzed RNA fraction in the same way as for the trichloroacetic acid , which
may cause solubili small - molecule fraction ( see above and 16.4.3 ) . zation of ...
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Contents
MORPHOLOGY | 5 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plate positive prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York