Manual of Methods for General Bacteriology |
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Page 222
... gene function allows both the identi- fication of that gene and , together with genetic mapping analyses , the localization of the gene on the chromosome . Second , the analysis of mutants ( strains that contain mutations ) which are ...
... gene function allows both the identi- fication of that gene and , together with genetic mapping analyses , the localization of the gene on the chromosome . Second , the analysis of mutants ( strains that contain mutations ) which are ...
Page 243
... gene transfer are known in bacteria . Transformation is a gene transfer process in which deoxyribonucleic acid ( DNA ) from one bacterium , the donor , is taken up by another bacterium , the recipient . A recipient cell that now ...
... gene transfer are known in bacteria . Transformation is a gene transfer process in which deoxyribonucleic acid ( DNA ) from one bacterium , the donor , is taken up by another bacterium , the recipient . A recipient cell that now ...
Page 256
... gene and to move that allele from one strain to another . Another useful method was developed by Hong and Ames ( 20 ) to induce mutations in unknown genes that are closely linked to known genes . By treating a transducing phage lysate ...
... gene and to move that allele from one strain to another . Another useful method was developed by Hong and Ames ( 20 ) to induce mutations in unknown genes that are closely linked to known genes . By treating a transducing phage lysate ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes