Manual of Methods for General Bacteriology |
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Page 57
... gradient - forming de- vices are available , but these are unnecessary since satisfactory gradients can be prepared by layering a series of sucrose solutions in a centri- fuge tube and allowing the gradient to " age " overnight in order ...
... gradient - forming de- vices are available , but these are unnecessary since satisfactory gradients can be prepared by layering a series of sucrose solutions in a centri- fuge tube and allowing the gradient to " age " overnight in order ...
Page 230
... Gradient Antibiotic - resistant mutants are easily iso- lated , and the resistances can often serve as convenient genetic markers for use in character- izing bacterial strains . In this example , a proce- dure for obtaining ...
... Gradient Antibiotic - resistant mutants are easily iso- lated , and the resistances can often serve as convenient genetic markers for use in character- izing bacterial strains . In this example , a proce- dure for obtaining ...
Page 297
... Gradient maker which gives linear gra- dients . The surface areas of beakers A and B are equal . position will change linearly from the limit in the second ( mixing ) reservoir to the limit in the first . The steepness of the gradient ...
... Gradient maker which gives linear gra- dients . The surface areas of beakers A and B are equal . position will change linearly from the limit in the second ( mixing ) reservoir to the limit in the first . The steepness of the gradient ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes