Manual of Methods for General Bacteriology |
From inside the book
Results 1-3 of 87
Page 60
... membrane is insoluble in Triton X - 100 while the cytoplas- mic membrane is completely solubilized . When cells are broken by shear forces , some of the cytoplasmic membrane becomes frag- mented extensively ( probably into short ...
... membrane is insoluble in Triton X - 100 while the cytoplas- mic membrane is completely solubilized . When cells are broken by shear forces , some of the cytoplasmic membrane becomes frag- mented extensively ( probably into short ...
Page 467
... membrane filter on distilled water so that the pores will be filled with water ( if the membrane is submersed immediately , air pockets will form and cause uneven filtration ) . 4. Place the wet membrane filter on the filtra- tion ...
... membrane filter on distilled water so that the pores will be filled with water ( if the membrane is submersed immediately , air pockets will form and cause uneven filtration ) . 4. Place the wet membrane filter on the filtra- tion ...
Page 468
... membranes ( about 5 μg of DNA per membrane ) from a 15 - cm membrane with a keysort punch ( 22.6.1 ) . 2. Preincubate the membranes at the incu- bation temperature of the experiment for 0.5 to 2 h . Agitate the membranes several times ...
... membranes ( about 5 μg of DNA per membrane ) from a 15 - cm membrane with a keysort punch ( 22.6.1 ) . 2. Preincubate the membranes at the incu- bation temperature of the experiment for 0.5 to 2 h . Agitate the membranes several times ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
7 other sections not shown
Other editions - View all
Common terms and phrases
Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes