Manual of Methods for General Bacteriology |
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Page 52
... method of cell breakage is the most important decision to be made in designing a cell fractionation scheme . The following questions must be kept in mind when choosing a breakage method : 1. Is the breakage method appropriate to the ...
... method of cell breakage is the most important decision to be made in designing a cell fractionation scheme . The following questions must be kept in mind when choosing a breakage method : 1. Is the breakage method appropriate to the ...
Page 413
... Method 1. Inoculate a nutrient agar slant ( see 20.3.25 ) or other medium lacking blood . After incubation , trickle 1 ml of 3 % hydrogen peroxide down the slant . Examine immediately and after 5 min for the evolution of bubbles , which ...
... Method 1. Inoculate a nutrient agar slant ( see 20.3.25 ) or other medium lacking blood . After incubation , trickle 1 ml of 3 % hydrogen peroxide down the slant . Examine immediately and after 5 min for the evolution of bubbles , which ...
Page 416
... method than 1B . Method 2. This method is more sensitive than Method 1A or 1B . Streak plates of agar medium supplemented with 0.4 % gelatin . Incu- bate at the optimum temperature for the organ- ism . Flood plates with gelatin ...
... method than 1B . Method 2. This method is more sensitive than Method 1A or 1B . Streak plates of agar medium supplemented with 0.4 % gelatin . Incu- bate at the optimum temperature for the organ- ism . Flood plates with gelatin ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes