Manual of Methods for General Bacteriology |
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Page 41
... pellet twice in Kellenberger Veronal - acetate buffer , centrifuging between washes . 4. Suspend the washed pellet in 1 % osmium tetroxide for 6 to 16 h at room temperature . ( The glutaraldehyde - fixed pellet may be en- robed in agar ...
... pellet twice in Kellenberger Veronal - acetate buffer , centrifuging between washes . 4. Suspend the washed pellet in 1 % osmium tetroxide for 6 to 16 h at room temperature . ( The glutaraldehyde - fixed pellet may be en- robed in agar ...
Page 394
... pellet and solution . Cell pellets contain not only cells but also interstitial ( intercellular ) space . The fractional volume of the interstitial space is generally estimated by use of polymeric solutes of high molecular weight which ...
... pellet and solution . Cell pellets contain not only cells but also interstitial ( intercellular ) space . The fractional volume of the interstitial space is generally estimated by use of polymeric solutes of high molecular weight which ...
Page 396
... pellets and incubation times . 5. Recentrifugation . After each mixture of pellet and test solution has been incubated , it should be centrifuged sufficiently to obtain a cell - free supernatant fluid for assay . The supernatant fluid ...
... pellets and incubation times . 5. Recentrifugation . After each mixture of pellet and test solution has been incubated , it should be centrifuged sufficiently to obtain a cell - free supernatant fluid for assay . The supernatant fluid ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes