Manual of Methods for General Bacteriology |
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Page 236
... phage lysate dilutions ( 10-5 , 10-6 , 10-7 ) in L broth . Incubate at 37 ° C for 20 min to allow phage adsorption . 3. Add 1.5 ml of LC top agar ( 13.9.17 ) at 55 ° C , mix by rotating the tube rapidly between the palms , and pour over ...
... phage lysate dilutions ( 10-5 , 10-6 , 10-7 ) in L broth . Incubate at 37 ° C for 20 min to allow phage adsorption . 3. Add 1.5 ml of LC top agar ( 13.9.17 ) at 55 ° C , mix by rotating the tube rapidly between the palms , and pour over ...
Page 249
... phage lysate is obtained either by spontaneous or induced liberation of phage from a lysogenized donor and never when the phage is propagated by the lytic productive cycle . On the other hand , with generalized transducing phages ...
... phage lysate is obtained either by spontaneous or induced liberation of phage from a lysogenized donor and never when the phage is propagated by the lytic productive cycle . On the other hand , with generalized transducing phages ...
Page 250
... phage . The donor parent , on the other hand , possesses the opposite allele which is most easily selected when transductants inherit that trait . General methods . The preparation of specialized transducing ly- sates requires the use ...
... phage . The donor parent , on the other hand , possesses the opposite allele which is most easily selected when transductants inherit that trait . General methods . The preparation of specialized transducing ly- sates requires the use ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
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