Manual of Methods for General Bacteriology: By Philipp Gerhardt, Editor-in-chief ; R.G.E. Murray, Editor, I. Morphology ... [et Al.].Philipp Gerhardt, American Society for Microbiology |
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Page 236
Mix 0.1 ml of this cell suspension with 0.1 ml of various phage lysate dilutions (10'
°, 10'°, 10'7) in L broth. Incubate at 37°C for 20 min to allow phage adsorption. 3.
Add 1.5 ml of LC top agar (13.9.17) at 55°C, mix by rotating the tube rapidly ...
Mix 0.1 ml of this cell suspension with 0.1 ml of various phage lysate dilutions (10'
°, 10'°, 10'7) in L broth. Incubate at 37°C for 20 min to allow phage adsorption. 3.
Add 1.5 ml of LC top agar (13.9.17) at 55°C, mix by rotating the tube rapidly ...
Page 249
TRAN SDUCTION Bacterial viruses are classified as either virulent, in which case
all infected cells die with liberation of new phage particles, or temperate, in which
the infected bacteria either lyse with liberation of new phage progeny or ...
TRAN SDUCTION Bacterial viruses are classified as either virulent, in which case
all infected cells die with liberation of new phage particles, or temperate, in which
the infected bacteria either lyse with liberation of new phage progeny or ...
Page 250
transducing phages can also pick up and transduce plasmid genetic information.
Such plasmid elements, upon introduction into the recipient host, become
established and commence to replicate in synchrony with the recipient
chromosome.
transducing phages can also pick up and transduce plasmid genetic information.
Such plasmid elements, upon introduction into the recipient host, become
established and commence to replicate in synchrony with the recipient
chromosome.
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Contents
MORPHOLOGY | 5 |
Light Microscopy R G E MURRAY AND C F Robinow 2 Specimen Preparation for Light Microscopy R G E MURRAY AND C F Robi | 17 |
Determinative Methods of Light Microscopy R N DoETscH | 21 |
Copyright | |
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Common terms and phrases
acetate Adjust agar allow amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacteriology bacterium biotin broth buffer carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described detergents determined dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter final first flask fraction g Distilled genetic glass glucose gradient gram-negative gram-positive grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid liter measure medium membrane metabolic method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein radioactivity reaction reagent remove sample slide sodium solution specific spheroplasts staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract
Bibliographic information
| Title | Manual of Methods for General Bacteriology: By Philipp Gerhardt, Editor-in-chief ; R.G.E. Murray, Editor, I. Morphology ... [et Al.]. |
| Editors | Philipp Gerhardt, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 22 Aug 2011 |
| ISBN | 0914826298, 9780914826293 |
| Length | 524 pages |
| Export Citation | BiBTeX EndNote RefMan |