Manual of Methods for General Bacteriology |
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Page 230
... plate technique is described which can be modified for the detection of many different types of mutants described above . In the second example , a simple filter disk assay is described for the induction and direct selection of revert ...
... plate technique is described which can be modified for the detection of many different types of mutants described above . In the second example , a simple filter disk assay is described for the induction and direct selection of revert ...
Page 233
... plate is reproduced on the replica plate , thus permitting the rapid examination of large numbers of individual colonies for mutant char- acteristics . A suitable dilution of mutagenized cells is first plated on the surface of a ...
... plate is reproduced on the replica plate , thus permitting the rapid examination of large numbers of individual colonies for mutant char- acteristics . A suitable dilution of mutagenized cells is first plated on the surface of a ...
Page 427
... plate wells simultaneously . ( A ) Stainless - steel tubing ; ( B ) 18 - gauge hypodermic needles brazed to tubing ; ( C ) microtiter plate with wells ; ( D ) Luer - Lok connector brazed to ... plate . Nine submaster plates can be prepared.
... plate wells simultaneously . ( A ) Stainless - steel tubing ; ( B ) 18 - gauge hypodermic needles brazed to tubing ; ( C ) microtiter plate with wells ; ( D ) Luer - Lok connector brazed to ... plate . Nine submaster plates can be prepared.
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes