Manual of Methods for General Bacteriology |
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Page 230
In the first example given below , the use of a simple gradient plate technique is
described which can be modified for the ... The ( 13.9.12 ) into sterile petri plates
100 mm in di resulting antibiotic gradient extends from a low conameter , and ...
In the first example given below , the use of a simple gradient plate technique is
described which can be modified for the ... The ( 13.9.12 ) into sterile petri plates
100 mm in di resulting antibiotic gradient extends from a low conameter , and ...
Page 236
Incubate the plates at 30 ° C for about 18 h ml of various phage lysate dilutions (
10-5 , 10-6 , or until transductant colonies are just visible to 10- ? ) in L broth .
Incubate at 37 ° C for 20 min to the naked eye . allow phage adsorption . 7.
Replica ...
Incubate the plates at 30 ° C for about 18 h ml of various phage lysate dilutions (
10-5 , 10-6 , or until transductant colonies are just visible to 10- ? ) in L broth .
Incubate at 37 ° C for 20 min to the naked eye . allow phage adsorption . 7.
Replica ...
Page 251
The lambda agar plates should be 100 mm test tubes and place these at 37 ° C .
equilibrated to room temperature or even pre 6. Dilute the lysate serially using 10
mM warmed , since use of " cold ” plates will cause the MgCl2 soft agar to ...
The lambda agar plates should be 100 mm test tubes and place these at 37 ° C .
equilibrated to room temperature or even pre 6. Dilute the lysate serially using 10
mM warmed , since use of " cold ” plates will cause the MgCl2 soft agar to ...
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Contents
MORPHOLOGY | 5 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method Microbiol microscopy mixture mutants needed obtained organisms oxygen plasmid plate positive prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Author | Philipp Gerhardt |
| Editor | Philipp Gerhardt |
| Contributors | R. G. E. Murray, Ralph N. Costilow, American Society for Microbiology, Willis A. Wood |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 22 Aug 2011 |
| ISBN | 0914826298, 9780914826293 |
| Length | 524 pages |
| Export Citation | BiBTeX EndNote RefMan |