Manual of Methods for General Bacteriology |
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Page 350
... procedure is based on the assay of thymine in hydrolysates of cell samples by gas chromatography . This procedure has the disadvantage that access to an appropriate gas chromatograph is required . If DNA assays are routinely performed ...
... procedure is based on the assay of thymine in hydrolysates of cell samples by gas chromatography . This procedure has the disadvantage that access to an appropriate gas chromatograph is required . If DNA assays are routinely performed ...
Page 468
... procedure . The direct binding procedure measures the ability of a labeled DNA to form duplexes with DNA preparations immobilized on membrane filters . Larger amounts of labeled DNA ( as com- pared to the free solution experiments ) are ...
... procedure . The direct binding procedure measures the ability of a labeled DNA to form duplexes with DNA preparations immobilized on membrane filters . Larger amounts of labeled DNA ( as com- pared to the free solution experiments ) are ...
Page 469
... procedure . In this procedure , unlabeled denatured DNA fragments are used to compete with denatured labeled DNA for duplex formation with DNA bound to the membrane filter . The competitor DNA preparations contain 1.5 mg of DNA per ml ...
... procedure . In this procedure , unlabeled denatured DNA fragments are used to compete with denatured labeled DNA for duplex formation with DNA bound to the membrane filter . The competitor DNA preparations contain 1.5 mg of DNA per ml ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes