Manual of Methods for General Bacteriology |
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Page 58
... proteins , and in the case of SDS this usually results in unfolding and irreversible de- naturation of proteins . Although ionic deter- gents can be removed by dialysis , this is not practical because of extensive protein binding ...
... proteins , and in the case of SDS this usually results in unfolding and irreversible de- naturation of proteins . Although ionic deter- gents can be removed by dialysis , this is not practical because of extensive protein binding ...
Page 323
... proteins . The open point represents the unknown protein . Chapter 17 Chemical Composition R. S. HANSON AND J. A.. Log electrophoretic mobility for each protein and standard run at several gel concentrations , i.e. , at different degrees ...
... proteins . The open point represents the unknown protein . Chapter 17 Chemical Composition R. S. HANSON AND J. A.. Log electrophoretic mobility for each protein and standard run at several gel concentrations , i.e. , at different degrees ...
Page 358
... Protein by Folin Reaction The most widely used colorimetric method for estimation of proteins is that of Lowry et al . ( 90 ) , using the Folin reaction . The blue color is due to the reaction of protein with copper ion in alkaline ...
... Protein by Folin Reaction The most widely used colorimetric method for estimation of proteins is that of Lowry et al . ( 90 ) , using the Folin reaction . The blue color is due to the reaction of protein with copper ion in alkaline ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes