Manual of Methods for General Bacteriology |
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Page 129
... remove any air bubbles under the seal . After the bacteria have grown , select a tube that contains well - isolated colonies , and remove the agar from the tube by first melting and discarding the paraffin seal and then inserting a ...
... remove any air bubbles under the seal . After the bacteria have grown , select a tube that contains well - isolated colonies , and remove the agar from the tube by first melting and discarding the paraffin seal and then inserting a ...
Page 331
... remove residual trichloroacetic acid , which may cause solubili- zation of some protein in the next step . Discard the filtrate . 3. Place the filter holder ( with the filter and suction flask ) and a test tube for collecting the ...
... remove residual trichloroacetic acid , which may cause solubili- zation of some protein in the next step . Discard the filtrate . 3. Place the filter holder ( with the filter and suction flask ) and a test tube for collecting the ...
Page 347
... Remove the upper aqueous layer and save it . Remove and discard the material at the interface . Add another 350 ml of water to the phenol layer , and sediment and mix thoroughly while the temper- ature is held at 65 to 68 ° C for 15 min ...
... Remove the upper aqueous layer and save it . Remove and discard the material at the interface . Add another 350 ml of water to the phenol layer , and sediment and mix thoroughly while the temper- ature is held at 65 to 68 ° C for 15 min ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes