Manual of Methods for General Bacteriology: By Philipp Gerhardt, Editor-in-chief ; R.G.E. Murray, Editor, I. Morphology ... [et Al.].Philipp Gerhardt, American Society for Microbiology |
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Page 129
Remove the gas cannula, replace the rubber stopper in the tube, and incubate
the culture in a vertical position. dium in roll tubes just before coating the walls of
the tubes. One disadvantage of diluting in agar media is that many of the isolated
...
Remove the gas cannula, replace the rubber stopper in the tube, and incubate
the culture in a vertical position. dium in roll tubes just before coating the walls of
the tubes. One disadvantage of diluting in agar media is that many of the isolated
...
Page 331
Wash the filter with 3 ml of ice-cold 70% ethanol to remove residual
trichloroacetic acid, which may cause solubilization of some protein in the next
step. Discard the filtrate. 3. Place the filter holder (with the filter and suction flask)
and a test ...
Wash the filter with 3 ml of ice-cold 70% ethanol to remove residual
trichloroacetic acid, which may cause solubilization of some protein in the next
step. Discard the filtrate. 3. Place the filter holder (with the filter and suction flask)
and a test ...
Page 347
Repeat the resuspension and centrifugation to remove medium constituents.
Lyophilize the bacteria in the final sediment, or store the packed cells in a freezer
until used. Thoroughly suspend 20 g (dry weight) of bacteria in 350 ml of water at
...
Repeat the resuspension and centrifugation to remove medium constituents.
Lyophilize the bacteria in the final sediment, or store the packed cells in a freezer
until used. Thoroughly suspend 20 g (dry weight) of bacteria in 350 ml of water at
...
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Contents
MORPHOLOGY | 5 |
Light Microscopy R G E MURRAY AND C F Robinow 2 Specimen Preparation for Light Microscopy R G E MURRAY AND C F Robi | 17 |
Determinative Methods of Light Microscopy R N DoETscH | 21 |
Copyright | |
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Common terms and phrases
acetate Adjust agar allow amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacteriology bacterium biotin broth buffer carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described detergents determined dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter final first flask fraction g Distilled genetic glass glucose gradient gram-negative gram-positive grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid liter measure medium membrane metabolic method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein radioactivity reaction reagent remove sample slide sodium solution specific spheroplasts staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract
Bibliographic information
| Title | Manual of Methods for General Bacteriology: By Philipp Gerhardt, Editor-in-chief ; R.G.E. Murray, Editor, I. Morphology ... [et Al.]. |
| Editors | Philipp Gerhardt, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 22 Aug 2011 |
| ISBN | 0914826298, 9780914826293 |
| Length | 524 pages |
| Export Citation | BiBTeX EndNote RefMan |