Manual of Methods for General Bacteriology |
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Page 93
... rumen fluid is prepared from rumen fluid freshly collected from a cow on an alfalfa hay - grain ration . Filter through gauze and allow to sediment ; siphon off the clear liquid and centrifuge at 14,000 rpm for 20 min ( 21 ) . ' For ...
... rumen fluid is prepared from rumen fluid freshly collected from a cow on an alfalfa hay - grain ration . Filter through gauze and allow to sediment ; siphon off the clear liquid and centrifuge at 14,000 rpm for 20 min ( 21 ) . ' For ...
Page 94
... rumen fluid - cellobiose agar or broth . Clarified rumen fluid is prepared from rumen fluid freshly collected from a cow on an alfalfa hay - grain ration . Filter through gauze and allow to sediment ; siphon off the clear liquid and ...
... rumen fluid - cellobiose agar or broth . Clarified rumen fluid is prepared from rumen fluid freshly collected from a cow on an alfalfa hay - grain ration . Filter through gauze and allow to sediment ; siphon off the clear liquid and ...
Page 137
... Rumen fluid ( Randolph Biologicals , Distilled water 1,000 ml Houston , Tex . ) 30 ml Cysteine hydrochloride 0.05 g Hemin solution ( see 20.3.28 ) 1.0 ml Adjust pH to 7.3 . Sterilize at 118 to 121 ° C for 15 min . Vitamin K , solution ...
... Rumen fluid ( Randolph Biologicals , Distilled water 1,000 ml Houston , Tex . ) 30 ml Cysteine hydrochloride 0.05 g Hemin solution ( see 20.3.28 ) 1.0 ml Adjust pH to 7.3 . Sterilize at 118 to 121 ° C for 15 min . Vitamin K , solution ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes