Manual of Methods for General Bacteriology |
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Page 30
... slide as in Gray's method , steps 1 and 2 . 2. Using a wax glass - marking pencil , draw a rectangle around the film . 3. Place 1 ml of the dye solution on the slide without allowing any to flow over the wax lines . Leave for 7 to 15 ...
... slide as in Gray's method , steps 1 and 2 . 2. Using a wax glass - marking pencil , draw a rectangle around the film . 3. Place 1 ml of the dye solution on the slide without allowing any to flow over the wax lines . Leave for 7 to 15 ...
Page 31
... slide does not show a pink tint . Store in the dark . 7. Wash the slide several times in a solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide ...
... slide does not show a pink tint . Store in the dark . 7. Wash the slide several times in a solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide ...
Page 431
... slide . Examine by oil immersion . Positive test : the capsules around the blue- stained cells will appear distinctly outlined . A comparison should always be made with a con- trol slide prepared using normal rabbit serum instead of ...
... slide . Examine by oil immersion . Positive test : the capsules around the blue- stained cells will appear distinctly outlined . A comparison should always be made with a con- trol slide prepared using normal rabbit serum instead of ...
Contents
Light Microscopy R G E MURRAY AND C F ROBINOW | 6 |
NOW | 17 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Copyright | |
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Academic Press acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium Biochem biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove sample slide sodium solution specific staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract Yes Yes