Manual of Methods for General Bacteriology: By Philipp Gerhardt, Editor-in-chief ; R.G.E. Murray, Editor, I. Morphology ... [et Al.].Philipp Gerhardt, American Society for Microbiology |
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Page 53
When procedures that involve conversion of cells to spheroplasts or protoplasts
in osmotically stabilized medium are employed, it is essential to determine the
percentage of conversion in a counting chamber rather than simply to examine
wet ...
When procedures that involve conversion of cells to spheroplasts or protoplasts
in osmotically stabilized medium are employed, it is essential to determine the
percentage of conversion in a counting chamber rather than simply to examine
wet ...
Page 55
Spheroplasts of enteric bacteria can be prepared by the procedure of Osbom and
Munson (8), as follows. Cultures are centrifuged and suspended in one-tenth of
the culture volume in cold 0.75 M sucrose in 10 mM Tris-acetate buffer, pH 7.8.
Spheroplasts of enteric bacteria can be prepared by the procedure of Osbom and
Munson (8), as follows. Cultures are centrifuged and suspended in one-tenth of
the culture volume in cold 0.75 M sucrose in 10 mM Tris-acetate buffer, pH 7.8.
Page 61
J. Bacteriol. l14:1I64-I176. Neu, H. C., and L. A. Heppel. 1965. The release of
enzymes from Escherichia coli by osmotic shock and during the formation of
spheroplasts. J. Biol. Chem. 240:3685-3692. Osborn, M. J ., and R. Munson.
1974.
J. Bacteriol. l14:1I64-I176. Neu, H. C., and L. A. Heppel. 1965. The release of
enzymes from Escherichia coli by osmotic shock and during the formation of
spheroplasts. J. Biol. Chem. 240:3685-3692. Osborn, M. J ., and R. Munson.
1974.
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Contents
MORPHOLOGY | 5 |
Light Microscopy R G E MURRAY AND C F Robinow 2 Specimen Preparation for Light Microscopy R G E MURRAY AND C F Robi | 17 |
Determinative Methods of Light Microscopy R N DoETscH | 21 |
Copyright | |
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Common terms and phrases
acetate Adjust agar allow amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacteriology bacterium biotin broth buffer carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described detergents determined dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter final first flask fraction g Distilled genetic glass glucose gradient gram-negative gram-positive grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid liter measure medium membrane metabolic method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein radioactivity reaction reagent remove sample slide sodium solution specific spheroplasts staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract
Bibliographic information
| Title | Manual of Methods for General Bacteriology: By Philipp Gerhardt, Editor-in-chief ; R.G.E. Murray, Editor, I. Morphology ... [et Al.]. |
| Editors | Philipp Gerhardt, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| Original from | the University of Michigan |
| Digitized | 22 Aug 2011 |
| ISBN | 0914826298, 9780914826293 |
| Length | 524 pages |
| Export Citation | BiBTeX EndNote RefMan |