Manual of Methods for General Bacteriology: By Philipp Gerhardt, Editor-in-chief ; R.G.E. Murray, Editor, I. Morphology ... [et Al.].Philipp Gerhardt, American Society for Microbiology |
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Page 96
2H2O (g) 0.1 0.25 FeSO4·7H2O (mg) 5.0 Amino acids Yes" Beef extract (g) 3.0
1.0 1.0 Yeast extract (g) 0.1-1.0 5.0 3.0 | 1.0 1.0 0.1 10 1-0. Acid-hydrolyzed
casein (e.g., Casamino Acids) (g) 0.1 Enzyme-hydrolyzed casein (e.g., Casitone)
(g) ...
2H2O (g) 0.1 0.25 FeSO4·7H2O (mg) 5.0 Amino acids Yes" Beef extract (g) 3.0
1.0 1.0 Yeast extract (g) 0.1-1.0 5.0 3.0 | 1.0 1.0 0.1 10 1-0. Acid-hydrolyzed
casein (e.g., Casamino Acids) (g) 0.1 Enzyme-hydrolyzed casein (e.g., Casitone)
(g) ...
Page 138
Standard Methods Agar (6) (BBL or Difco) Pancreatic digest of casein, USP 5.0 g
Yeast extract 2.5g Glucose 1.0 g Agar 15.0 g Distilled water 1,000 ml Adjust pH to
7.0. Boil to dissolve agar. Sterilize at 121°C for 15 min. 8.5.49. Succinate-Salts ...
Standard Methods Agar (6) (BBL or Difco) Pancreatic digest of casein, USP 5.0 g
Yeast extract 2.5g Glucose 1.0 g Agar 15.0 g Distilled water 1,000 ml Adjust pH to
7.0. Boil to dissolve agar. Sterilize at 121°C for 15 min. 8.5.49. Succinate-Salts ...
Page 140
Wiley and Stokes Alkaline Medium (82) Yeast extract 20.0 g (NH4)2SO4 10.0 g
Tris(hydroxymethyl)aminomethane (Tris buffer) 15.7g Distilled water 1,000 ml
Prepare stock solutions of each ingredient separately and sterilize by autoclaving
.
Wiley and Stokes Alkaline Medium (82) Yeast extract 20.0 g (NH4)2SO4 10.0 g
Tris(hydroxymethyl)aminomethane (Tris buffer) 15.7g Distilled water 1,000 ml
Prepare stock solutions of each ingredient separately and sterilize by autoclaving
.
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Contents
MORPHOLOGY | 5 |
Light Microscopy R G E MURRAY AND C F Robinow 2 Specimen Preparation for Light Microscopy R G E MURRAY AND C F Robi | 17 |
Determinative Methods of Light Microscopy R N DoETscH | 21 |
Copyright | |
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acetate Adjust agar allow amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacteriology bacterium biotin broth buffer carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described detergents determined dilution Dissolve distilled water electron microscopy endospores enzyme Escherichia coli ethanol film filter final first flask fraction g Distilled genetic glass glucose gradient gram-negative gram-positive grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid liter measure medium membrane metabolic method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein radioactivity reaction reagent remove sample slide sodium solution specific spheroplasts staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract