Gene Cloning: An Introduction |
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Page 57
... DNA molecules is described on p . 77 . 4.1.3 Polymerases DNA polymerases are enzymes that synthesize a new strand of DNA complementary to an existing DNA or RNA template ( Figure 4.5 ( a ) ) . Most polymerases can function only if the ...
... DNA molecules is described on p . 77 . 4.1.3 Polymerases DNA polymerases are enzymes that synthesize a new strand of DNA complementary to an existing DNA or RNA template ( Figure 4.5 ( a ) ) . Most polymerases can function only if the ...
Page 58
... polymerase I -A - T - G - C - A - A - T - G - C - A - T- -T - A - C - G G ... DNA Figure 4.5 The reactions catalysed by DNA polymerases . ( a ) ... DNA modifying enzymes There are numerous enzymes that modify 58 Manipulation of purified DNA.
... polymerase I -A - T - G - C - A - A - T - G - C - A - T- -T - A - C - G G ... DNA Figure 4.5 The reactions catalysed by DNA polymerases . ( a ) ... DNA modifying enzymes There are numerous enzymes that modify 58 Manipulation of purified DNA.
Page 228
... DNA molecule , a single gene for instance , is copied many times by a DNA polymerase enzyme ( see Figure 1.4 ) . This may seem a ... POLYMERASE CHAIN REACTION IN OUTLINE. The polymerase chain reaction The polymerase chain reaction in outline.
... DNA molecule , a single gene for instance , is copied many times by a DNA polymerase enzyme ( see Figure 1.4 ) . This may seem a ... POLYMERASE CHAIN REACTION IN OUTLINE. The polymerase chain reaction The polymerase chain reaction in outline.
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast