Gene Cloning: An Introduction |
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Page 236
... product is studied in various ways in order to gain information about the DNA molecule that acted as the original template . Although a wide range of techniques have been devised for studying PCR ... PCR Size markers 2 PCR Restrict 2 3 236 ...
... product is studied in various ways in order to gain information about the DNA molecule that acted as the original template . Although a wide range of techniques have been devised for studying PCR ... PCR Size markers 2 PCR Restrict 2 3 236 ...
Page 237
An Introduction Terence A. Brown. R 1 PCR Size markers 2 PCR Restrict 2 3 PCR 3 In some cases , agarose gel electrophoresis is used not only to determine if the PCR experiment has worked ... PCR product is of course PCR in more detail 237.
An Introduction Terence A. Brown. R 1 PCR Size markers 2 PCR Restrict 2 3 PCR 3 In some cases , agarose gel electrophoresis is used not only to determine if the PCR experiment has worked ... PCR product is of course PCR in more detail 237.
Page 238
... product then the fragments can be ligated into a vector and examined by any ... PCR products . From an examination of Figure 11.2 it might be imagined that fragments ... PCR Denature Bottom strand primers 238 The polymerase chain reaction.
... product then the fragments can be ligated into a vector and examined by any ... PCR products . From an examination of Figure 11.2 it might be imagined that fragments ... PCR Denature Bottom strand primers 238 The polymerase chain reaction.
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast