Gene Cloning: An Introduction |
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Page 90
... ampicillin into a form that is non - toxic to the bacterium , and a sec- ond set of genes that code for enzymes that detoxify tetracycline . Uptake of pBR322 can be detected because the E. coli cells are transformed from ampicillin- and ...
... ampicillin into a form that is non - toxic to the bacterium , and a sec- ond set of genes that code for enzymes that detoxify tetracycline . Uptake of pBR322 can be detected because the E. coli cells are transformed from ampicillin- and ...
Page 95
... ampicillin , but sensitive to tetracycline ( ampRtetS ) . Screening for pBR322 recombinants is performed in the follow- ing way . After transformation the cells are plated on to ampicillin medium and incubated until colonies appear ...
... ampicillin , but sensitive to tetracycline ( ampRtetS ) . Screening for pBR322 recombinants is performed in the follow- ing way . After transformation the cells are plated on to ampicillin medium and incubated until colonies appear ...
Page 96
An Introduction Terence A. Brown. ( a ) Colonies on ampicillin medium Figure 5.8 Screening for pBR322 recombinants by insertional inactiva- tion of the tetracycline resistance gene . ( a ) Cells are plated on to ampicillin agar : all the ...
An Introduction Terence A. Brown. ( a ) Colonies on ampicillin medium Figure 5.8 Screening for pBR322 recombinants by insertional inactiva- tion of the tetracycline resistance gene . ( a ) Cells are plated on to ampicillin agar : all the ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast