Gene Cloning: An Introduction |
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Page 200
... analysis and genetic fingerprinting . 9.3.1 Restriction fragment length polymorphism analysis If two DNA molecules are very similar , but have a few small dif- ferences in their nucleotide sequences , then the fact that they are not ...
... analysis and genetic fingerprinting . 9.3.1 Restriction fragment length polymorphism analysis If two DNA molecules are very similar , but have a few small dif- ferences in their nucleotide sequences , then the fact that they are not ...
Page 330
... analysis 230 , 236-7 , 247-8 restriction analysis 74-6 retardation analysis 213–14 RFLP analysis 200 running a gel 69–70 separation of chromosomes 187-8 Southern transfer 184 strand separation 196 Gel retardation 213-14 , 317g ...
... analysis 230 , 236-7 , 247-8 restriction analysis 74-6 retardation analysis 213–14 RFLP analysis 200 running a gel 69–70 separation of chromosomes 187-8 Southern transfer 184 strand separation 196 Gel retardation 213-14 , 317g ...
Page 333
... analysis 248-9 , 322g Random priming 171-2 , 322g RAPD analysis 248–9 , 322g Rat 301t Recombinant 94 , 322g Recombinant DNA molecule , defi- nition 4 , 322g Recombinant DNA technology , definition 4 , 322g Recombinant insulin 275-8 ...
... analysis 248-9 , 322g Random priming 171-2 , 322g RAPD analysis 248–9 , 322g Rat 301t Recombinant 94 , 322g Recombinant DNA molecule , defi- nition 4 , 322g Recombinant DNA technology , definition 4 , 322g Recombinant insulin 275-8 ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast