Gene Cloning: An Introduction |
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Page 17
An Introduction Terence A. Brown. Donor cell Conjugative plasmid Recipient cell Figure 2.4 Plasmid transfer by con- jugation between bacterial cells . The donor and recipient cells attach to each other by a pilus , a hollow appendage ...
An Introduction Terence A. Brown. Donor cell Conjugative plasmid Recipient cell Figure 2.4 Plasmid transfer by con- jugation between bacterial cells . The donor and recipient cells attach to each other by a pilus , a hollow appendage ...
Page 27
... cell DNA will often be required as a source of material from which to obtain genes to be cloned . Total cell DNA may be DNA from a culture of bacteria , from a plant , from animal cells , or from any other type of organism that is being ...
... cell DNA will often be required as a source of material from which to obtain genes to be cloned . Total cell DNA may be DNA from a culture of bacteria , from a plant , from animal cells , or from any other type of organism that is being ...
Page 30
An Introduction Terence A. Brown. Figure 3.2 Estimation of bacterial cell number by measurement of opti- cal density . ( a ) A sample of the cul- ture is placed in a glass cuvette and light with a wavelength of 600 nm shone through . The ...
An Introduction Terence A. Brown. Figure 3.2 Estimation of bacterial cell number by measurement of opti- cal density . ( a ) A sample of the cul- ture is placed in a glass cuvette and light with a wavelength of 600 nm shone through . The ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast