Gene Cloning: An Introduction |
From inside the book
Results 1-3 of 20
Page 28
... cell DNA from a culture of bacteria . 1 A culture of bacteria is grown and then harvested 2 The cells are removed and broken to give a cell extract Centrifugation Cell extract Bacterial culture Pellet of cells 4 The DNA is concentrated ...
... cell DNA from a culture of bacteria . 1 A culture of bacteria is grown and then harvested 2 The cells are removed and broken to give a cell extract Centrifugation Cell extract Bacterial culture Pellet of cells 4 The DNA is concentrated ...
Page 32
... cell debris Centrifuge Cell extract Cell extract DNA , RNA , protein Cell debris Having lysed the cells , the final step in preparation of a cell extract is removal of insoluble cell debris . Components such as par- tially digested cell ...
... cell debris Centrifuge Cell extract Cell extract DNA , RNA , protein Cell debris Having lysed the cells , the final step in preparation of a cell extract is removal of insoluble cell debris . Components such as par- tially digested cell ...
Page 35
... cell cultures are becom- ing increasingly important in biology . The major ... extract are protein , DNA and RNA , so phenol extraction and / or protease ... cell extract the nucleic acid - CTAB complex precipitates , leaving carbohydrate ...
... cell cultures are becom- ing increasingly important in biology . The major ... extract are protein , DNA and RNA , so phenol extraction and / or protease ... cell extract the nucleic acid - CTAB complex precipitates , leaving carbohydrate ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
13 other sections not shown
Other editions - View all
Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast