Gene Cloning: An Introduction |
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... Cloning vectors for E. coli 107 6.1 Cloning vectors based on E. coli plasmids 107 6.2 Cloning vectors based on M13 bacteriophage 6.3 Cloning vectors based on λ bacteriophage 115 121 6.4 λ and other high capacity vectors enable genomic ...
... Cloning vectors for E. coli 107 6.1 Cloning vectors based on E. coli plasmids 107 6.2 Cloning vectors based on M13 bacteriophage 6.3 Cloning vectors based on λ bacteriophage 115 121 6.4 λ and other high capacity vectors enable genomic ...
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An Introduction Terence A. Brown. Cloning vectors for E. coli The basic experimental techniques involved in gene cloning have now been described . In Chapters 3 , 4 and 5 ... Cloning vectors for E coli Cloning vectors based on E coli ...
An Introduction Terence A. Brown. Cloning vectors for E. coli The basic experimental techniques involved in gene cloning have now been described . In Chapters 3 , 4 and 5 ... Cloning vectors for E coli Cloning vectors based on E coli ...
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... cloning vectors are based on a λ - like phage called C31 . Most of these vectors are very similar to E. coli ... cloning vectors . II . A multi - purpose cloning system . Gene , 2 , 95-113 - pBR322 . Melton , D. A. , Krieg , P. A. ...
... cloning vectors are based on a λ - like phage called C31 . Most of these vectors are very similar to E. coli ... cloning vectors . II . A multi - purpose cloning system . Gene , 2 , 95-113 - pBR322 . Melton , D. A. , Krieg , P. A. ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast