Gene Cloning: An Introduction |
From inside the book
Results 1-3 of 22
Page 95
... colonies appear ( Figure 5.8 ( a ) ) . All of these colonies are transformants ( remember , untransformed cells are amps so do not produce colonies on the selective medium ) , but ( a ) The normal vector molecule Ampicillin resistance ...
... colonies appear ( Figure 5.8 ( a ) ) . All of these colonies are transformants ( remember , untransformed cells are amps so do not produce colonies on the selective medium ) , but ( a ) The normal vector molecule Ampicillin resistance ...
Page 96
... colonies . ( b ) The colonies are replica - plated on to tetracycline medium . ( c ) The colonies that grow on tetracycline medium are amp tet and therefore non - recombinants . Recombinants ( ampotets ) do not grow , but their position ...
... colonies . ( b ) The colonies are replica - plated on to tetracycline medium . ( c ) The colonies that grow on tetracycline medium are amp tet and therefore non - recombinants . Recombinants ( ampotets ) do not grow , but their position ...
Page 181
... colonies that bind the label are detected by autoradiography , or ( a ) Immunoscreening Colonies Lyse cells ( chloroform ) Membrane Protein A binds to antibody ( b ) The resulting autoradiograph Add specific antibody Antibody attaches ...
... colonies that bind the label are detected by autoradiography , or ( a ) Immunoscreening Colonies Lyse cells ( chloroform ) Membrane Protein A binds to antibody ( b ) The resulting autoradiograph Add specific antibody Antibody attaches ...
Contents
Further reading | 12 |
Purification of DNA from living cells | 27 |
Further reading | 50 |
Copyright | |
18 other sections not shown
Other editions - View all
Common terms and phrases
able acid addition amount amplified analysis animal attached bacterial bacteriophage BamHI bands Biotechnology carried cDNA cell Chapter chromosome cloned gene cloning vectors coded coli colonies complete construction containing copy correct culture deletion desired detected developed direct disease DNA fragment DNA molecule EcoRI electrophoresis ends engineering enzyme example experiment expression Figure gene cloning genetic genome glycosylation host human hybridization identified important individual infection inserted involved labelled ligated marker means medium method molecular mRNA mutation natural needed normal nucleotide obtained occur organisms origin phage plant plaques plasmid polymerase position possible preparation present primers probe problem promoter protein purified reaction recombinant DNA molecule region removed replication resistance result segment selection separated sequence single single-stranded specific strand structure synthesis T-DNA techniques tion transcription transfer transformed translation types usually virus yeast