Gene Cloning: An Introduction |
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Page 213
An Introduction Terence A. Brown. Control sequences 200 bp Gene Promoter Figure 10.6 Possible positions for control sequences in the region upstream of a gene . regulatory protein to the control sequence , is a problem for genet- ics and ...
An Introduction Terence A. Brown. Control sequences 200 bp Gene Promoter Figure 10.6 Possible positions for control sequences in the region upstream of a gene . regulatory protein to the control sequence , is a problem for genet- ics and ...
Page 215
... control sequence from the degradative action of an endonuclease such as DNase I ( Figure 10.9 ) . This phenomenon ... control sequence are absent . Their absence shows up on the autoradi- ograph as a ' footprint ' , clearly seen in ...
... control sequence from the degradative action of an endonuclease such as DNase I ( Figure 10.9 ) . This phenomenon ... control sequence are absent . Their absence shows up on the autoradi- ograph as a ' footprint ' , clearly seen in ...
Page 219
... sequence has been removed , a decrease will indicate removal of an activator or enhancer , and a change in tissue - specificity ( as shown in Figure 10.13 ) will pinpoint a tissue - responsive control sequence . Seed - specific control ...
... sequence has been removed , a decrease will indicate removal of an activator or enhancer , and a change in tissue - specificity ( as shown in Figure 10.13 ) will pinpoint a tissue - responsive control sequence . Seed - specific control ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast