Gene Cloning: An Introduction |
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Page 75
... digestion in one step , if both enzymes have similar requirements for pH , Mg2 + concentration , etc. Alternatively , the two digestions may have to be carried out one after the other , adjusting the reac- tion mixture after the first ...
... digestion in one step , if both enzymes have similar requirements for pH , Mg2 + concentration , etc. Alternatively , the two digestions may have to be carried out one after the other , adjusting the reac- tion mixture after the first ...
Page 76
... digestion . Partial digestion Enzyme Fragment sizes ( kb ) Kpnl - limiting conditions 1.5 , 17.0 , 18.5 , 30.0 , 31.5 , 48.5 Conclusions : 48.5 kb fragment = uncut 2 . 1.5 , 17.0 and 30.0 kb fragments are products of complete digestion ...
... digestion . Partial digestion Enzyme Fragment sizes ( kb ) Kpnl - limiting conditions 1.5 , 17.0 , 18.5 , 30.0 , 31.5 , 48.5 Conclusions : 48.5 kb fragment = uncut 2 . 1.5 , 17.0 and 30.0 kb fragments are products of complete digestion ...
Page 333
... digestion 75 frequency of restriction sites 65 function 61 importance in gene cloning 6-7 , 60-1 overlapping restriction frag- ments 199 partial digestion 75 , 130 performing a restriction digest 65-8 recognition sequences 62-3 , 63t ...
... digestion 75 frequency of restriction sites 65 function 61 importance in gene cloning 6-7 , 60-1 overlapping restriction frag- ments 199 partial digestion 75 , 130 performing a restriction digest 65-8 recognition sequences 62-3 , 63t ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast