Gene Cloning: An Introduction |
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Page 256
... expression in E. coli . Promoter Ribosome binding site Terminator Gene Point at which ribosome binds to mRNA -DNA ... vector in such a way that it is placed under control of a set of E. coli expression signals . If this can be achieved ...
... expression in E. coli . Promoter Ribosome binding site Terminator Gene Point at which ribosome binds to mRNA -DNA ... vector in such a way that it is placed under control of a set of E. coli expression signals . If this can be achieved ...
Page 259
... expression vector . It may therefore be possible to extend the regulation to the expression vector , so that the chemical that induces or represses the gene normally controlled by the promoter is also able to regu- late expression of ...
... expression vector . It may therefore be possible to extend the regulation to the expression vector , so that the chemical that induces or represses the gene normally controlled by the promoter is also able to regu- late expression of ...
Page 260
An Introduction Terence A. Brown. Figure 12.7 Four promoters often used in expression vectors . The lac and trp promoters are shown upstream for the genes that they ... expression vector requires 260 Production of protein from cloned genes.
An Introduction Terence A. Brown. Figure 12.7 Four promoters often used in expression vectors . The lac and trp promoters are shown upstream for the genes that they ... expression vector requires 260 Production of protein from cloned genes.
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast