Gene Cloning: An Introduction |
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Page 256
... foreign gene is inactive in E. coli quite sim- ply because the bacterium does not recognize its expression sig- nals . A solution to this problem would be to insert the foreign gene into the vector in such a way that it is placed under ...
... foreign gene is inactive in E. coli quite sim- ply because the bacterium does not recognize its expression sig- nals . A solution to this problem would be to insert the foreign gene into the vector in such a way that it is placed under ...
Page 261
... Foreign gene RT Cassette PR Foreign gene inserted -into the cassette With some cassette vectors the cloning site is not immediately adjacent to the ribosome binding sequence but instead is preceded by a segment from the beginning of an ...
... Foreign gene RT Cassette PR Foreign gene inserted -into the cassette With some cassette vectors the cloning site is not immediately adjacent to the ribosome binding sequence but instead is preceded by a segment from the beginning of an ...
Page 262
... gene PR PR Unique restriction site T Insert a foreign gene Expression Foreign gene T Correct fusion ...... GGA GCT ATA E.coli TTA ...... Foreign gene will be translated Incorrect fusion ...... GGA GCATA TTA ...... Foreign gene will not ...
... gene PR PR Unique restriction site T Insert a foreign gene Expression Foreign gene T Correct fusion ...... GGA GCT ATA E.coli TTA ...... Foreign gene will be translated Incorrect fusion ...... GGA GCATA TTA ...... Foreign gene will not ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast