Gene Cloning: An Introduction |
From inside the book
Results 1-3 of 29
Page 69
... electrophoresis DNA ( b ) Gel electrophoresis Electrophorese Buffer DNA migrates towards the anode , but little separation into size classes occurs DNA , loaded into a well cut out of the gel Electrophorese Gel DNA separates into bands ...
... electrophoresis DNA ( b ) Gel electrophoresis Electrophorese Buffer DNA migrates towards the anode , but little separation into size classes occurs DNA , loaded into a well cut out of the gel Electrophorese Gel DNA separates into bands ...
Page 213
... gel electrophoresis , as it has a lower mobility than the uncomplexed DNA molecule ( Figure 10.7 ) . The procedure is referred to as gel retardation . For gel retardation to be of value the region containing the con- trol sequence must ...
... gel electrophoresis , as it has a lower mobility than the uncomplexed DNA molecule ( Figure 10.7 ) . The procedure is referred to as gel retardation . For gel retardation to be of value the region containing the con- trol sequence must ...
Page 214
... gel electrophoresis . 1 2 Lane 1 : DNA fragment Lane 2 : DNA fragment + bound protein Agarose gel Figure 10.8 Carrying out a gel retar- dation experiment . retardation be able to pinpoint it exactly . A more precise technique is ...
... gel electrophoresis . 1 2 Lane 1 : DNA fragment Lane 2 : DNA fragment + bound protein Agarose gel Figure 10.8 Carrying out a gel retar- dation experiment . retardation be able to pinpoint it exactly . A more precise technique is ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
13 other sections not shown
Other editions - View all
Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast