Gene Cloning: An Introduction |
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Page 206
An Introduction Terence A. Brown. 10 Studying gene expression All genes have to be expressed in order to function . The first step in expression is transcription of the gene into a complementary RNA strand ( Figure 10.1 ( a ) ) . For some ...
An Introduction Terence A. Brown. 10 Studying gene expression All genes have to be expressed in order to function . The first step in expression is transcription of the gene into a complementary RNA strand ( Figure 10.1 ( a ) ) . For some ...
Page 217
... expression of a gene should result in that gene being expressed at a higher level . Similarly , tis- sue - specific control sequences can be ... gene . Control sequences Promoter Control Studying the regulation of gene expression 217.
... expression of a gene should result in that gene being expressed at a higher level . Similarly , tis- sue - specific control sequences can be ... gene . Control sequences Promoter Control Studying the regulation of gene expression 217.
Page 259
... gene off Transcripts important in induction and repression lie in the region surround- ing the promoter and are therefore also present in an expression vector . It may therefore be possible to extend the regulation to the expression ...
... gene off Transcripts important in induction and repression lie in the region surround- ing the promoter and are therefore also present in an expression vector . It may therefore be possible to extend the regulation to the expression ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast