Gene Cloning: An Introduction |
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Page 184
... hybridization that obscures the specific hybridization signal . Instead the DNA bands in the agarose gel are transferred to a nitrocellulose or nylon membrane , providing a much ' cleaner ' environment for the hybridization experiment ...
... hybridization that obscures the specific hybridization signal . Instead the DNA bands in the agarose gel are transferred to a nitrocellulose or nylon membrane , providing a much ' cleaner ' environment for the hybridization experiment ...
Page 330
... hybridization 191 , 317g Fluorochrome 191 Flush end 314g Footprinting 214-15 , 317g Forensic analysis 244 F plasmid ... Hybridization analysis abundancy probing 174 by electron microscopy 208-9 chromosome walking 191-2 colony ...
... hybridization 191 , 317g Fluorochrome 191 Flush end 314g Footprinting 214-15 , 317g Forensic analysis 244 F plasmid ... Hybridization analysis abundancy probing 174 by electron microscopy 208-9 chromosome walking 191-2 colony ...
Page 331
An Introduction Terence A. Brown. RFLP analysis 200 Southern hybridization 184-6 , 236 , 290 , 305 uses 174-9 Hybridization probe 168 , 318t Hybrid protein 261-4 , 277 , 278-9 Hybrid - release translation 220 , 318g Hygromycin ...
An Introduction Terence A. Brown. RFLP analysis 200 Southern hybridization 184-6 , 236 , 290 , 305 uses 174-9 Hybridization probe 168 , 318t Hybrid protein 261-4 , 277 , 278-9 Hybrid - release translation 220 , 318g Hygromycin ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast