Gene Cloning: An Introduction |
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Page 170
... hybrid ( b ) A stable hybrid Short non - complementary regions do not affect overall stability ( c ) A DNA - RNA hybrid DNA RNA 8.4.2 Colony and plaque hybridization probing Hybridization probing can be used to identify recombinant DNA ...
... hybrid ( b ) A stable hybrid Short non - complementary regions do not affect overall stability ( c ) A DNA - RNA hybrid DNA RNA 8.4.2 Colony and plaque hybridization probing Hybridization probing can be used to identify recombinant DNA ...
Page 174
... hybridization probing Clearly the success of colony or plaque hybridization as a means of identifying a particular recombinant clone hangs on the availability of a DNA molecule that can be used as a probe . This probe must share at ...
... hybridization probing Clearly the success of colony or plaque hybridization as a means of identifying a particular recombinant clone hangs on the availability of a DNA molecule that can be used as a probe . This probe must share at ...
Page 330
... hybridization 191 , 317g Fluorochrome 191 Flush end 314g Footprinting 214-15 ... probing 176-9 , 318g HindIII 63t HinfI 63 , 63t HIV 285t Homology 318g ... Hybridization analysis abundancy probing 174 by electron microscopy 208-9 ...
... hybridization 191 , 317g Fluorochrome 191 Flush end 314g Footprinting 214-15 ... probing 176-9 , 318g HindIII 63t HinfI 63 , 63t HIV 285t Homology 318g ... Hybridization analysis abundancy probing 174 by electron microscopy 208-9 ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast