Gene Cloning: An Introduction |
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Page 213
... Identifying protein - binding sites on a DNA molecule A control sequence is a region of DNA that can bind a regulatory ... identified by gel electrophoresis , as it has a lower mobility than the uncomplexed DNA molecule ( Figure 10.7 ) ...
... Identifying protein - binding sites on a DNA molecule A control sequence is a region of DNA that can bind a regulatory ... identified by gel electrophoresis , as it has a lower mobility than the uncomplexed DNA molecule ( Figure 10.7 ) ...
Page 287
... Identification of the mutation present in a defective gene can be used to devise a screening programme so that the mutant gene can be identified in individuals who are carriers or who have not yet developed the disease . Carriers can ...
... Identification of the mutation present in a defective gene can be used to devise a screening programme so that the mutant gene can be identified in individuals who are carriers or who have not yet developed the disease . Carriers can ...
Page 289
... identified on chromosome 17 , reduced the size of the BRCA1- containing region from 20 Mb down to just 600 kb ( Figure 13.10 ) . ( b ) Identification of a candidate gene for BRCA1 Now that the relevant region had been narrowed down to ...
... identified on chromosome 17 , reduced the size of the BRCA1- containing region from 20 Mb down to just 600 kb ( Figure 13.10 ) . ( b ) Identification of a candidate gene for BRCA1 Now that the relevant region had been narrowed down to ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast