Gene Cloning: An Introduction |
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Page 206
... introns and con- siderable interest centres on their structure and possible function . In addition to introns , the exact locations of the start and end points of transcription are important . Most transcripts are copies not only of the ...
... introns and con- siderable interest centres on their structure and possible function . In addition to introns , the exact locations of the start and end points of transcription are important . Most transcripts are copies not only of the ...
Page 207
... introns Introns Figure 10.1 Some fundamentals of gene expression . rRNA , tRNA DNA molecule Transcription Primary RNA transcript still contains introns Processing Mature mRNA- - no introns Translation Protein ( c ) RNA transcripts ...
... introns Introns Figure 10.1 Some fundamentals of gene expression . rRNA , tRNA DNA molecule Transcription Primary RNA transcript still contains introns Processing Mature mRNA- - no introns Translation Protein ( c ) RNA transcripts ...
Page 209
... introns , can be compared with the gene sequence to locate the introns with precision . Introns -RNA DNA Figure 10.3 The appearance under the electron microscope of a DNA - RNA hybrid formed between a gene containing an intron and its ...
... introns , can be compared with the gene sequence to locate the introns with precision . Introns -RNA DNA Figure 10.3 The appearance under the electron microscope of a DNA - RNA hybrid formed between a gene containing an intron and its ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast