Gene Cloning: An Introduction |
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Page 12
... Molecular Approach , 2nd edn , Chapman & Hall , London , UK – an introduction to genetics and molecular biology . - Kendrew , A. ( ed . ) ( 1994 ) The Encyclopedia of Molecular Biology . Blackwell Science , Oxford , UK - an outstanding ...
... Molecular Approach , 2nd edn , Chapman & Hall , London , UK – an introduction to genetics and molecular biology . - Kendrew , A. ( ed . ) ( 1994 ) The Encyclopedia of Molecular Biology . Blackwell Science , Oxford , UK - an outstanding ...
Page 87
... molecular biologist to create novel recombinant DNA molecules . The next step in a gene cloning experiment is to introduce these molecules into living cells , usually bacteria , which will then grow and divide to produce clones ( Figure ...
... molecular biologist to create novel recombinant DNA molecules . The next step in a gene cloning experiment is to introduce these molecules into living cells , usually bacteria , which will then grow and divide to produce clones ( Figure ...
Page 159
... molecules and transformation of living cells are used in the real world to obtain clones of specific genes and to provide information of importance to molecular biology and biotechnology . In this part of the book , the role and ...
... molecules and transformation of living cells are used in the real world to obtain clones of specific genes and to provide information of importance to molecular biology and biotechnology . In this part of the book , the role and ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast